High frequency in vitro propagation of M × M60, a cherry rootstock: the effects of culture media and growth regulators

Authors

1 Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran.

2 Stone Fruit Research Group, Seed and Plant Improvement Research Institute of Karaj Mahdasht Road, P.O. Box 31585-4119, Karaj , Iran.

3 Department of Biotechnology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran.

Abstract

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and ME supplemented with 0, 0.5, 0.7, 1 mg L-1 of BAP. As a result, shoot number, average shoot length node number and internode length (except leaf area) were significantly affected by the interaction of medium with BAP level. The largest number of shoots was obtained in DKW (10.3 shoots/explants), ME (9.1 shoots/explants) and QL (9.1 shoots/explants) containing 0.7 mg L-1 BAP. The results showed that DKW with 0.7 mg L-1 BAP had the highest productivity compared to other treatments. For rooting, in vitro proliferated and elongated shoots were planted on LS, MS and ½ MS medium containing 0, 0.5, 1 and 1.5 mg L-1 of IBA. High rooting percentage, root length and root number were obtained on ½ MS supplemented with 1 mg L-1 IBA. The in vitro plantlets were transferred into the soil and the survival rate ex vitro was 95%.

Keywords

References

Aghaye R. N. M., Yadollahi A., Moeini A., and Sepahvand S. (2013). In vitro culture of Gisela 6 semi-dwarf rootstock. Journal of Environmental Sciences, 7: 57-64.
Ahmad T., and Laghari M. (2004). Effect of different auxins on in vitro rooting of peach rootstock GF677. Sarhad Journal of Agriculture (Pakistan).
Andreu P., and Marín J. A. (2005). In vitro culture establishment and multiplication of the Prunus rootstock ‘Adesoto 101’( P. insititia L.) as affected by the type of propagation of the donor plant and by the culture medium composition. Scientia Horticulturae, 106: 258-267.
Borkowska B. (1984). Micropropagation of sour cherry, cultivar Schattenmorelle. International Workshop on Improvement of Sweet and Sour Cherry Varieties and Rootstocks 169.
Bošnjak A. M., Kereša S., Jerčić I. H., and Barić M. (2012). The effect of cytokinin type and explant orientation on axillary shoot proliferation and in vitro rooting of ‘Gisela 5’cherry rootstock. Journal of Food, Agriculture & Environment, 10: 616-620.
Canli F., and Tian L. (2008). In vitro shoot regeneration from stored mature cotyledons of sweet cherry (Prunus avium L.) cultivars. Scientia Horticulturae, 116: 34-40.
Cos J., Frutos D., Sÿnchez M., Rodriguez J., and Carrillo A. (2002). Determination of the optimal culture medium and growth regulator concentration for the in vitro proliferation stage of the peach-almond hybrid Mayor®. I International Symposium on Rootstocks for Deciduous Fruit Tree Species 658.
Dalzotto A. and Docampo D. (1997). Micropropagation of rootstock from the Marianna 2624 plum (Prunus cerasifera X P. munsoniana) and the Pixy plum (P. insititia L) under controlled conditions. Phyton-International Journal of Experimental Botany, 60: 127-135.
Doric D., Ognjanov V., Ljubojevic M., Barac G., Dulic J., Pranjic A., and Dugalic K. (2014). Rapid propagation of sweet and sour cherry rootstocks. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42: 488.
Dradi G., Vito G., and Standardi A. (1993). In vitro mass propagation of eleven Prunus mahaleb ecotypes. II International Cherry Symposium 410.
Drew R. A., Simpson B. W., and Osborne W. J. (1991). Degradation of exogenous indole-3-butyric acid and riboflavin and their influence on rooting response of papaya in vitro. Plant Cell, Tissue and Organ Culture, 26: 29-34.
Driver J. A., and Kuniyuki A. H. (1984). In vitro propagation of paradox walnut rootstock [Juglans hindsii X Juglans regia, tissue culture]. HortScience (USA), 19:507–509.
Ďurkovič J. (2006). Rapid micropropagation of mature wild cherry. Biologia Plantarum, 50: 733-736.
Fallahpour M., Miri S. M., and Bouzari N. (2015). In vitro propagation of ‘Gisela 5’rootstock as affected by mineral composition of media and plant growth regulators. Journal of Horticultural Research, 23: 57-64.
Fuernkranz H., Nowak C., and Maynard C. (1990). Light effects on in vitro adventitious root formation in axillary shoots of mature Prunus serotina. Physiologia Plantarum 80: 337-341.
Ganji Moghaddam A., and Abdollahzadeh Gonabadi A. (2008). Fruit trees guide (apple, pear, sweet cherry and prune. Iran, Gholami.
George E. F., Hall M. A., and De Klerk G. -J. (2008). The components of plant tissue culture media I: macro-and micro-nutrients. Springer.
George E. F., Hall M. A., and De Klerk G.-J. (2008). Micropropagation: uses and methods. Springer.
Gorst J., Slaytor M., and De Fossard R. (1983). The effect of indole-3-butyric acid and riboflavin on the morphogenesis of adventitious roots of Eucalyptus ficifolia F. Muell. grown in vitro. Journal of Experimental Botany, 34: 1503-1515.
Hammatt N., and Grant N. (1997). Micropropagation of mature British wild cherry. Plant Cell, Tissue and Organ Culture, 47: 103-110.
Hammatt N., and Grant N. (1998). Shoot regeneration from leaves of Prunus serotina Ehrh.(black cherry) and P. avium L.(wild cherry). Plant Cell Reports, 17: 526-530.
Hausman J. (1993). Changes in peroxidase activity, auxin level and ethylene production during root formation by poplar shoots raised in vitro. Plant Growth Regulation, 13: 263-268.
Hossini A., Moghadam E., and Anahid S. (2010). Effects of media cultures and plant growth regulators in micro propagation of Gisela 6 rootstock. Annals of Biological Research, 1: 135-141.
Hrotkó K. (2016). Potentials in Prunus mahaleb L. for cherry rootstock breeding. Scientia Horticulturae, 205: 70-78.
Hrotkó K., and Füzesséry A. (1993). Effect of rootstock on the branching and quality of cherry trees in the nursery. II International Cherry Symposium 410.
Hrotkó K., Magyar L., Simon G., and Hanusz B. (1996). Effect of rootstocks and interstocks on growth and yield of sweet cherry trees. VI International Symposium on Integrated Canopy, Rootstock, Environmental Physiology in Orchard Systems 451.
Hrotkó K., and Simon G. (1993). Effect of rootstock on the growth and productivity of cherry trees. II International Cherry Symposium 410.
Kalinina A., and Brown D. C. (2007). Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator. Plant Cell Reports, 26: 927-935.
Linsmaier E. M., and Skoog F. (1965). Organic growth factor requirements of tobacco tissue cultures. Physiologia Plantarum, 18: 100-127.
Loreti F., Morini S., and Pasqualetto P. (1987). Effect of alternating temperature during proliferation and rooting stages of GF 655/2 and GF 677 shoots cultured "in vitro". International Symposium on Vegetative Propagation of Woody Species 227.
Maene L., and Debergh P. (1982). Rooting of tissue cultured  plants under in vivo conditions. In Vitro Culture, XXI I-HC, 131: 201-208.
Mahdavian M., Bouzari N., and Abdollahi H. (2011). Effects of media and plant growth regulators on micropropagation of a dwarfing cherry rootstock (PHL-A). Biharean Biologist, 5: 86-90.
Mansseri-Lamrioui A., Louerguioui A., and Abousalim A. (2009). Effect of the medium culture on the micro cutting of material resulting from adult cuttings of wild cherry trees (Prunus avium L.) and of in vitro germination. European Journal of Scientific Research, 25: 345-352.
Mansseri-Lamrioui A., Louerguioui A., Bonaly J., Yakoub-Bougdal S., Allili N., and Gana-Kebbouche S. (2013). Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration. African Journal of Biotechnology, 10: 8613-8624.
Matt A., and Jehle J. A. (2005). In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.). Plant Cell Reports, 24: 468-476.
Muna A.-S., Ahmad A.-K., Mahmoud K., and Abdul-Rahman K. (1999). In vitro propagation of a semi-dwarfing cherry rootstock. Plant Cell, Tissue and Organ Culture, 59: 203-208.
Murashige T., and Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
Nissen S. J., and Sutter E. G. (1990). Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience, 25: 800-802.
Pruski K. (2007). Tissue culture propagation of Mongolian cherry (Prunus fruticosa L.) and Nanking cherry (Prunus tomentosa L.). Protocols for Micropropagation of Woody Trees and Fruits, Springer: 391-407.
Pruski K. W., Lewis T., Astatkie T., and Nowak J. (2000). Micropropagation of Chokecherry and Pincherry cultivars. Plant Cell, Tissue and Organ Culture, 63: 93-100.
Quoirin M., and Lepoivre P. (1977). Improved media for in vitro culture of Prunus sp. Symposium on Tissue Culture for Horticultural Purposes 78.
Rom C., and Ferree D. (1984). The influence of summer pruning current-season shoots on growth, floral bud development, and winter injury of mature peach trees. HortScience, 19: 543-545.
Ružić D. V., and Vujović T. (2008). The effects of cytokinintypes and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). Horticultural Science, 35: 12-21.
Saponari M., Bottalico G., and Savino V. (1999). In vitro propagation of Prunus mahaleb and its sanitation from prune dwarf virus. Advances in Horticultural Science, 13: 56-60.
Sarropoulou V., Dimassi-Theriou K., and Therios I. (2013). Effects of exogenous l-arginine on in vitro rooting, chlorophyll, carbohydrate, and proline concentrations in the sweet cherry rootstock M× M 14 (Prunus avium L.× Prunus mahaleb L.). Plant Biotechnology Reports, 7: 457-465.
Sarropoulou V. N., Therios I. N., and Dimassi‐Theriou K. N. (2012). Melatonin promotes adventitious root regeneration in in vitro shoot tip explants of the commercial sweet cherry rootstocks CAB‐6P (Prunus cerasus L.), Gisela 6 (P. cerasus× P. canescens), and MxM 60 (P. avium× P. mahaleb). Journal of Pineal Research, 52: 38-46.
Sedlak J., Paprstein F., and Erbenova M. (2008). In vitro propagation of the P-HL dwarfing sweet cherry rootstocks. Acta horticulturae.
Singh S., Sundouri A., Sharma M., Srivastava K., and Dar H. (2010). Proliferation and rooting efficiency studies in sour cherry (Prunus cerasus) using in vitro techniques. The Journal of Horticultural Science, 5: 48-52.
Šiško M. (2011). In vitro propagation of Gisela 5 (Prunus cerasus× P. canescens). Agricultura (Slovenia), 8: 31-34.
Van der Krieken W. M., Breteler H., Visser M. H. and Mavridou D. (1993). The role of the conversion of IBA into IAA on root regeneration in apple: introduction of a test system. Plant Cell Reports, 12: 203-206.
Vujović T., Ružić D., and Cerović R. (2012). In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. Hortscience (Prague), 39: 101-107.
Vujovic T., Ruzic D., Cerovic R., and Djordjevic M. (2009). The influence of imidazole fungicide on multiplication in vitro of low vigorous sweet cherry rootstock Gizela 6. Vocarstvo (Serbia).
Zilkah S., Faingersh E., and Rotbaum A. (1991). In vitro propagation of three MXM (Prunus avium X P. mahaleb) cherry rootstocks. II International Symposium on Propagation of Ornamental Plants 314.
Iranian Journal of Genetics and Plant Breeding
Volume 4, Issue 2 - Serial Number 8
October 2015
Pages 28-36

Files

History

  • Receive Date: 11 July 2016
  • Accept Date: 11 July 2016

Share

How to cite

Statistics