High frequency in vitro propagation of M × M60, a cherry rootstock: the effects of culture media and growth regulators


1 Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran.

2 Stone Fruit Research Group, Seed and Plant Improvement Research Institute of Karaj Mahdasht Road, P.O. Box 31585-4119, Karaj , Iran.

3 Department of Biotechnology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran.


Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and ME supplemented with 0, 0.5, 0.7, 1 mg L-1 of BAP. As a result, shoot number, average shoot length node number and internode length (except leaf area) were significantly affected by the interaction of medium with BAP level. The largest number of shoots was obtained in DKW (10.3 shoots/explants), ME (9.1 shoots/explants) and QL (9.1 shoots/explants) containing 0.7 mg L-1 BAP. The results showed that DKW with 0.7 mg L-1 BAP had the highest productivity compared to other treatments. For rooting, in vitro proliferated and elongated shoots were planted on LS, MS and ½ MS medium containing 0, 0.5, 1 and 1.5 mg L-1 of IBA. High rooting percentage, root length and root number were obtained on ½ MS supplemented with 1 mg L-1 IBA. The in vitro plantlets were transferred into the soil and the survival rate ex vitro was 95%.


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