Screening lentil germplasm for resistance against Ascochyta lentis under field and controlled conditions

Document Type : Research paper

Authors

1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran.

2 Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.

3 Dryland Agricultural Research Institute (DARI), Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran.

Abstract

Lentil (Lens culinaris) is the third most important grain legume in the world after chickpeas and peas. Ascochyta blight (AB) caused by Ascochyta lentis, is one of the most damaging diseases of this crop worldwide. In this study response of 169 lentil genotypes against AB was appraised in the greenhouse and field conditions based on randomized complete block design (RCBD). Field screening was carried out in the growing season of 2019-2020 at Shirvan-Chardavol Agricultural Research Station, Ilam province, Iran. Conidial suspension with concentration of 1×106 spore per mL was used for artificial infection in the greenhouse condition. Analysis of variance discovered significant differences among studied genotypes in response to the pathogen. There were no detected resistant genotypes among the studied lentil germplasm against AB in either environments. In the field condition, the majority of genotypes were identified as resistant while in the greenhouse condition most of the genotypes retained susceptible responses. Regarding AUDPC and disease severity characteristics, genotypes “G121”, “G132”, and “G139” were recognized as resistant in both field and greenhouse coditions. Classification of lentil genotypes by using Ward algorithm in both conditions resulted in 4 heterotic groups. The identified resistant genotypes can be useful in the breeding program of interested susceptible lentil genotypes by the backcross approach. Also, the identified heterotic groups can consider in parental selection for mapping QTLs related to resistance to the disease.

Keywords


Ahmad M., Russell A. C., and McNeil D. L. (1997). Identification and genetic characterization of different resistance sources to ascochyta blight within the genus Lens. Euphytica, 97: 311-315. DOI: https://doi.org/10.1023/A:1003095423132.
Bedasa T. (2021). Identification of lentil genotypes for resistance to ascochyta blight (Ascochyta lentis). Journal of Plant Pathology & Microbiology, 12: p007.
Baker R. J. (1986). Selection indices in plant breeding. CRC Press, pp. 232. DOI: https://doi.org/10.1201/9780429280498.
Bayaa B., Erskine W., and Hamdi A. (1994). Response of wild lentil to Ascochyta fabae f sp lentis from Syria. Genetic Resources and Crop Evolution, 41: 61-65. DOI: https://doi.org/10.1007/BF00053049.
Goh T. K. (1999). Single-spore isolation using a hand-made glass needle. Fungal Diversity, 2: 47-63.
Gossen B. D., and Morrall R. A. A. (1984). Seed quality loss at harvest due to Ascochyta blight of lentil. Canadian Journal Plant Pathology, 6: 233-237. DOI: https://doi.org/10.1080/07060668409501559.
Hussain S., Ali A., Zahid M. A., and Iqbal S. M. (2008). Multiple disease resistance in lentil germplasm from Pakistan. Pakistan Journal of Botany, 40: 897-903.
Iqbal A., Khalil I. A., Ateeq N., and Khan M. S. (2006). Nutritional quality of important food legumes. Food Chemistry, 97: 331-335. DOI: https://doi.org/10.1016/j.foodchem.2005.05.011.
Kaiser W. J., Wang B. C., and Rogers J. D. (1997). Ascochyta fabae and A. lentis: host specificity, teleomorphs (Didymella), hybrid analysis, and taxonomic status. Plant Disease, 81: 809-816. DOI: https://doi.org/10.1094/PDIS.1997.81.7.809.
McIntosh R. A. (1988). Genetical strategies for disease control. In: Miller T. E., and Koebner R. M. D. (Eds.). Proceedings of the Seventh International Wheat Genetics Symposium,Vol. 1, Institute of Plant Science Research, Cambridge, U.K., 39-44.
Nasir M., and Bretag T. W. (1997). Prevalence of Ascochyta fabae f.sp. lentis on lentil seed from Victoria, Australia. Australasian Plant Pathology, 26: 117-120. DOI: https://doi.org/10.1071/AP97018.
Parh D. K. (1998). Interspecific mapping of the Ascochyta gene(s) of lentil (Lens culinaris Medikus) using RAPD technology. MS Thesis, Lincoln University, Canterbury, New Zealand.
Sakr B. (1994). Inheritance and linkage of morphological markers and resistance to Ascochyta blight in lentil. PhD Thesis, Washington State Univ., Pullman, WA, USA.
Sandoval-Islas J. S., Broers L. H. M., Mora-Aguilera G., Parlevliet J. E., Osada-Kawasoe S., and Vivar, H. E. (2007). Quantitative resistance and its components in 16 barley cultivars to yellow rust, Puccinia striiformis f. sp. hordei. Euphytica, 153: 295-308. DOI: https://doi.org/10.1007/s10681-006-9236-y.
Sheikh M. I., Abdul G., Shaukat A., and Iftikhar A. (2010). Identification of resistant source of Ascochyta blight from local lentil germplasm. Pakistan Journal of Phytopathology, 22: 29-33.
Tay J. (1989). Inheritance of resistance to Ascochyta blight of lentil. MSc Thesis, Dept of Crop Science and Plant Ecology, Univ. of Saskatchewan, Saskatoon, Canada.
Tullu A., Banniza S., Taran B., Warkentin T., and Vandenberg A. (2010). Sources of resistance to ascochyta blight in wild species of lentil (Lens culinaris Medik.). Genetic Resource Crop Environment, 7: 1053-1063. DOI: https://doi.org/10.1007/s10722-010-9547-7.
Vandenberg A., Kiehn F. A., Vera C., Gaudiel R., Buchwaldt L., Dueck S., Wahab J., and Slinkard A. E. (2002). CDC Robin lentil. Canadian Journal of Plant Science, 82: 111-112. DOI: 10.4141/P01-003.
Ye G., Mcneil D. L., and Hill G. D. (2003). Inheritance of foliar resistance to ascochyta blight in lentil (Lensculinaris). New Zealand Journal of Crop and Horticultural Science, 31: 77-83. DOI: 10.1080/01140671.2003.9514239.
Ye G., McNeil D. L., and Hill G. D. (2000). Two major genes confer Ascochyta blight resistance in Lens orientalis. New Zealand Plant Protection, 53: 109-113. DOI: 10.30843/nzpp.2000.53.3622.
Ye G., McNeil D. L., and Hill G. D. (2001). Inheritance of resistance to Ascochyta blight in lentil. New Zealand Plant Protection, 54: 198-201. DOI: 10.30843/nzpp.2001.54.3722.
Zewdie A., and Gemachu B. (2020). Field evaluation of lentil germplasms for their resistance to Ascochyta blight (Ascochyta lentis) under field conditions. African Journal of Agricultural Research, 16: 1432-1436. DOI: 10.5897/AJAR2019.14609.