بهینه‌سازی القای ریشة‌‌ مویین در کاسنی (Cichorium intybus L.) و تاثیر نانوذرات بر تجمع متابولیت‌های ثانویه در آن

نوع مقاله : Research Paper

نویسندگان

گروه علوم باغبانی، دانشکده‌ی کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران، کدپستی: 11367-56199.

چکیده

کشت ریشه‌های مویین روشی مؤثر برای تولید متابولیت ثانویة گیاهی است؛ زیرا ریشه‌های مویین از پایداری ژنتیکی و بیولوژیکی بیشتری برخوردارند و قادر به تولید متابولیت بدون نیاز به هورمون هستند. در این میان، کاسنی نیز از جمله گیاهان دارویی ارزشمند و شامل ترکیبات دارویی مهمی است. در این جستار، القای ریشه‌های مویین توسط سویه‌های اگروباکتریوم رایزوژنز در ریزنمونه‌های برگ و دمبرگ بررسی شد. در آزمایش دوم، تأثیر نانو ذرات اکسید آهن و روی بر میزان تجمع زیست‌توده و تولید متابولیت ثانویه بررسی شد. نتایج حاصل نشان داد که بیشترین درصد القای ریشه‌های مویین (60 درصد) توسط سویةA4  در ریزنمونه‌های برگی بدست آمد. همچنین نانوذرات اکسید آهن و ریشه‌های مویین حاصل از سویة ATCC15834  بهترین تأثیر را در افزایش وزن تر (53/4 گرم)، خشک (4/0 گرم)، شاخص رشدی (41/24) و محتوای فنول کل (65/4 میلی‌گرم برگرم وزن خشک ریشه) متابولیت ثانویه داشته‌اند. بطور کلی، نوع سویه‌ و ریزنمونه‌ نقشی مهم در القای ریشه‌های مویین داشته و نانوذرات اکسید آهن و سویة ATCC15834  بیشترین کارایی را در رشد ریشه‌ها و تولید متابولیت ثانویه داشته است.

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