GF677 rootstock (peach ×almond) is an important rootstock for peach. It was produced by Bernhard in French at Grand Farad Research Centre in 1965. The present study focused on the effect of some PGRs on propagation and root induction on GNH medium as part of providing an applicable protocol for micro-propagation of mentioned rootstock. Results indicated that BAP at concentration of 0.5 mg/l plus 0.1 mg/l IBA produced the highest normal auxiliary shoots. Media supplemented with ZR produced only single shoots. The greatest rate of rooting (up to 40%) was obtained from induction medium containing 2 mg/l of IBA.