Detection of somaclonal variation in plants regenerated from different tissues of strawberry (Fragaria x ananassa) using ISSR marker

Document Type: Research paper

Authors

1 Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, P. O. Box: 578, Sari, Iran.

2 Department of Horticulture science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

3 Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Abstract

Production of genetically and phenotypically stable plantlets is the main purpose in commercial strawberry tissue culture. In this study, different tissues of Fragaria ananassa cv. Camarosa including stipule, apical meristem, leaf and petiole were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzyladenine (BA) (0.5, 1, 2, 3 mg/L) and indole-3-butyric acid (IBA) (0.1 and 0.5 mg/L). For apical meristem explants, the best regeneration rate (7.6 shoots per each explant) was obtained in the medium containing 1 mg/L BA and 0.1 mg/L IBA. Whereas stipule explants showed the highest regeneration rate in the medium containing 2 mg/L BA and 0.1 mg/L IBA. For leaf and petiole explants, the medium containing 2 mg/L BA and 0.5 mg/L IBA had the best hormonal combination. To determine the genetic variation, micropropagated plants from different tissue (after 8 subcultures) were analyzed by the inter-simple sequence repeats (ISSR) molecular marker. Among the 18 pre-selected primers, 10 displayed clear, reproducible and informative bands. A total of 88 distinct bands with a polymorphic rate of 46% were produced in the molecular profile of different explants. The highest and the lowest similarity values to maternal plants belonged to stipule and petiole explants with a similarity index of 0.738 and 0.645, respectively. Vitroplants derived from stipule and apical meristem showed the highest genetic stability with respect to maternal plants. With respect to genetic stability and regeneration rate, apical meristem can be recommended as suitable explants with the highest genetic fidelity. The findings of this study could be applied for commercial scale multiplication of strawberry, and also demonstrate that ISSR markers are eligible for detection of somaclonal variations.

Keywords


Agarwal M., Shrivastava N., and Padh H. (2008). Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Reports, 27: 617-631.

Babaei A., Nematzadeh G., and Hashemi H. (2011). An evaluation of genetic differentiation in rice mutants using semi-random markers and morphological characteristics. Australian Journal of Crop Science, 5: 1715.

Bacchetta L., Aramini M., Bernardini C., and Sivakumar L. (2008). High-Tech Production of Bioactive α-Tocopherol from Corylus avellana Adventitious Roots by Bioreactor Culture. Acta Hortic, 845: 713-716. DOI: 10.17660/ActaHortic.2009.845.112.

Bhatia R., Singh K., Jhang T., and Sharma T. (2009). Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers. Scientia Horticulturae, 119(20):211-218.

Biswas M., Dutt M., Roy U., Islam R., and Hossain M. (2009). Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Scientia Horticulturae, 122: 409-416.

Debnath S. C., and Teixeira da Silva. (2007). Strawberry culture in vitro: applications in genetic transformation and biotechnology. Fruit, Vegetable and Cereal Science and Biotechnology, 1: 1-12.

Durner E. F., Poling E. B., and Maas J. L. (2002). Recent advances in strawberry plug transplant technology. HortTechnology, 12: 545-550.

Gantait S., Mandal N., Bhattacharyya S., and Das P. K. (2010). Sustainable in vitro propagation and clonal fidelity in strawberry. The International Journal of Developmental Biology, 4: 19-25.

Ghasemi Y., Beaicknejad S., Sohrevardi F., Sharifani M., Amiri E., and Nematzadeh G. A. (2015). Adventitious Shoot and Root Regeneration of Wild Strawberry (F. viridis Duch.) by Means of Tissue Culture Medium Optimization. Biological Forum - An International Journal, 7: 436-441.

Gimenez M. D., Yañez-Santos A. M., Paz R. C., Quiroga M. P., Marfil C. F., Conci V. C., and García-Lampasona S. C. (2016). Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation. Plant cell reports, 35: 129-141.

Haghpanah M., Kazemitabar S. K., Hashemi S. H., and Alavi S. M. (2016). Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations. Journal of Plant Molecular Breeding, 4: 10-16.

Hashemi-Petroudi S. H., Maibody S. A. M. M., Nematzadeh G. A., and Arzani A. (2010). Semi-random PCR markers for DNA fingerprinting of rice hybrids and theirs corresponding parents. African Journal of Biotechnology, 9: 979-985.

Hashemi-Petroudi S. H., Nematzadeh G., Askari H., and Ghahary S. (2014). Involvement of Cytosine DNA methylation in different developmental stages of Aeluropus littoralis. Journal of Plant Molecular Breeding, 2: 56-67.

Hashemi S. H., Nematzadeh G., Askari H., and Ghasemi Y. (2012). Pattern of DNA cytosine methylation in Aeluropus littoralis during temperature stress. Journal of Plant Molecular Breeding, 1: 16-24.

Jahani M., Nematzadeh G., Dolatabadi B., Hashemi S. H., and Mohammadi-Nejad G. (2014). Identification and validation of functional markers in a global rice collection by association mapping. Genome, 57: 355-362.

Joshi P., and Dhawan V. (2007). Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. Biologia Plantarum, 51: 22-26.

Ko C., Al-Abdulkarim A., Al-Jowid S., and Al-Baiz A. (2009). An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry. African Journal of Biotechnology, 8: 2611-2615.

Krishna H., Alizadeh M., Singh D., Singh U., Chauhan N., Eftekhari M., and Sadh R. K. (2016). Somaclonal variations and their applications in horticultural crops improvement. 3 Biotech, 6: 54.

Lakshmanan V., Reddampalli Venkataramareddy S., and Neelwarne B. (2007). Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers. Electronic Journal of Biotechnology, 10: 106-113.

Leroy X., Leon K., Charles G., and Branchard M. (2000). Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs. Plant Cell Reports, 19: 1102-1107.

Litz R. E. (2005). Biotechnology of fruit and nut crops (CABI Chapter (Chapter number).

Mahjoob B., Zarini H., Hashemi S., and Shamasbi F. (2016). Comparison of ISSR, IRAP and REMAP markers for assessing genetic diversity in different species of Brassica sp. Russian Journal of Genetics, 52: 1272-1281.

Murashige T., and Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.

Naing A. H., Kim S. H., Chung M. Y., Park S. K., and Kim C. K. (2019). In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars. Plant methods, 15: 36.

Negi D., and Saxena S. (2010). Ascertaining clonal fidelity of tissue culture raised plants of Bambusa balcooa Roxb. using inter simple sequence repeat markers. New Forests, 40: 1-8.

Noormohammadi Z., Kangarloo-Haghighi B., Sheidai M., Farahani F., and Ghasemzadeh-Baraki S. (2014). Genetic stability versus somaclonal variation in tissue culture regenerated olive plants (Olea europea cv. Kroneiki). European Journal of Experimental Biology, 4: 135-142.

Olhoft P. M., and Phillips R. L. (2018). Genetic and epigenetic instability in tissue culture and regenerated progenies. Plant Responses to Environmental Stresses, Routledge, 111-148.

Passey A., Barrett K., and James D. (2003). Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria×ananassa Duch.) using a range of explant types. Plant Cell Reports, 21: 397-401.

Renau-Morata B., Nebauer S., Arrillaga I., and Segura J. (2005). Assessments of somaclonal variation in micropropagated shoots of Cedrus: consequences of axillary bud breaking. Tree Genetics & Genomes, 1: 3-10.

Rout G., Das P., Goel S., and Raina S. (1998). Determination of genetic stability of micropropagated plants of ginger using random amplified polymorphic DNA (RAPD) markers. Botanical Bulletin of Academia Sinica, 39: 23-27.

Sahijram L., Soneji J. R., and Bollamma K. (2003). Invited Review: Analyzing somaclonal variation in micropropagated bananas (Musa spp.). In Vitro Cellular & Developmental Biology 39: 551-556.

Sahraroo A., Zarei A., and Babalar M. (2019). In vitro regeneration of the isolated shoot apical meristem of two commercial fig cultivars ‘Sabz’and ‘Jaami-e-Kan’. Biocatalysis and Agricultural Biotechnology, 17: 743-749.

Salari R., Saravi A. T., and Kamali-Aliabad K. (2018). Determination of the optimized culture medium and growth conditions for micropropagation of Persian oak (Quercus brantii L.). Iranian Journal of Genetics and Plant Breeding, 7: 1-8.

Samantaray S., and Maiti S. (2010). An assessment of genetic fidelity of micropropagated plants of Chlorophytum borivilianum using RAPD markers. Biologia plantarum, 54: 334-338.

Sarkar S., and Jha S. (2017). Morpho-histological characterization and direct shoot organogenesis in two types of explants from Bacopa monnieri on unsupplemented basal medium. Plant Cell, Tissue and Organ Culture, 130: 435-441.

Shenoy V., and Vasil I. (1992). Biochemical and molecular analysis of plants derived from embryogenic tissue cultures of napier grass (Pennisetum purpureum K. Schum). Theoretical and Applied Genetics, 83: 947-955.

Shingote P. R., Mithra S. A., Sharma P., Devanna N. B., Arora K., Holkar S. K., Sharma T. (2019). LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane. Molecular Breeding, 39: 25.

Solano M. C. P., Ruíz J. S., Arnao M. T. G., Castro O. C., Tovar M. E. G., and Bello J. J. B. (2019). Evaluation of in vitro shoot multiplication and ISSR marker based assessment of somaclonal variants at different subcultures of vanilla (Vanilla planifolia Jacks). Physiology and Molecular Biology of Plants, 25: 561-567.

Tahmasi S., Garoosi G., Ahmadi J., and Farjaminezhad R. (2017). Effect of salicylic acid on stevioside and rebaudioside: A production and transcription of biosynthetic genes in in vitro culture of Stevia rebaudiana. Iranian Journal of Genetics and Plant Breeding, 6: 1-8.

Thorat A. S., Sonone N. A., Choudhari V. V., Devarumath R. M., and Babu K. H. (2018). Plant regeneration from direct and indirect organogenesis and assessment of genetic fidelity in Saccharum officinarum using DNA-based markers. Bioscience Biotechnology Research Communications, 11: 60-69.

Venkatachalam L., Sreedhar R., and Bhagyalakshmi N. (2007). Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers. Journal of Plant Growth Regulation, 51: 193-205.

Viswavidyalaya M. (2011). Advances in micropropagation of selected aromatic plants: a review on vanilla and strawberry. American Journal of Biochemistry and Molecular Biology, 1: 1-19.