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Iranian Journal of Genetics and Plant Breeding
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Razi, Z., Rahnama, H. (2012). Factors affecting delivery of DREB1A gene in maize B73 split-seeds via biolistic system. Iranian Journal of Genetics and Plant Breeding, 1(2), 36-43.
Zahra Razi; Hasan Rahnama. "Factors affecting delivery of DREB1A gene in maize B73 split-seeds via biolistic system". Iranian Journal of Genetics and Plant Breeding, 1, 2, 2012, 36-43.
Razi, Z., Rahnama, H. (2012). 'Factors affecting delivery of DREB1A gene in maize B73 split-seeds via biolistic system', Iranian Journal of Genetics and Plant Breeding, 1(2), pp. 36-43.
Razi, Z., Rahnama, H. Factors affecting delivery of DREB1A gene in maize B73 split-seeds via biolistic system. Iranian Journal of Genetics and Plant Breeding, 2012; 1(2): 36-43.

Factors affecting delivery of DREB1A gene in maize B73 split-seeds via biolistic system

Article 6, Volume 1, Issue 2, Summer and Autumn 2012, Page 36-43  XML PDF (2.57 MB)
Authors
Zahra Razi; Hasan Rahnama*
.
Abstract
Immature embryos as a choice tissue for genetic transformation of maize have a few limitations, such as genotype dependence, time-consuming and requiring a well-equipped greenhouse for access, at any time. In the present study, the split-seed explants were used for genetic transformation of maize, B73 line. The transformation of maize split-seed explants from the inbred line B73, for resistance to abiotic stresses was carried out via particle bombardment using pCA-35S-DREB construct, carrying Arabidopsis transcription factor DREB1A under the control of the CaMV 35S promoter and the selectable marker bar. For the optimization of gene-gun transformation parameters, we used two distances, 6 and 9 cm, one or double shooting and pre-treatment culturing of explants. The results showed that double shooting in 9 cm under pre -treatment conditions can improve transient expression of GUS gene in split-seed explants. PCR analysis confirmed the presence of DREB1A and bar genes in one out of 11 transformants. The results showed that split-seeds of B73 line can be used as a suitable explant for maize transformation.
Keywords
DREB1A; gus; Maize; Split-seed; Transformation
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