The effects of different concentrations of Zinc oxide nano particles (ZnONP; 0, 10, 25, 50, 75 and 100 mg L-1) and Humic acid (HA; 0, 50, 100, 500 and 1000 mg L-1) on root length and number, leaf length and number, bulb diameter, and chlorophyll, total phenol, anthocyanin and flavonoid contents were examined under in vitro conditions. Total phenol, flavonoid and anthocyanin contents were significantly (P ≤ 0.01) affected. The maximum phenolic content of plantlets was obtained in the medium containing 75 mg L-1 ZnONP. The highest anthocyanin content was observed in plantlets treated with HA at 100 mg L-1. The highest flavonoid content measured at 270, 300 and 330 nm wavelengths were obtained with ZnONP at 25 mg L-1, ZnONP at 25 mg L-1and HA at 100 mg L-1, respectively. Explants treated with HA produced the highest root length, leaf length and number, bulb number and chlorophyll content in the media containing 500, 50, 50, 50 and 500 mg L-1 HA, respectively. However, the media containing 50, 50, 50, 75 and 50 mg L-1 ZnONP produced the highest root length, leaf length and number, bulb number and chlorophyll, respectively.