Efficiency of anchored and non-anchored ISSR markers to estimate genetic diversity among bread wheat cultivars


1 Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran, Postal code: 53751-71379.

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.


DNA markers are integrally connected to the success of molecular breeding and are fundamentally required by breeders to be able to, a. identify new gene sources in the available biodiversity, b. select parents in order to increase heterosis, c. decrease the number of backcross generations for gene introgression breeding programs, and d. carry out marker-assisted selection (MAS). The present research was conducted to examine the efficiency of anchored and non-anchored ISSR molecular markers for grouping 20 bread wheat cultivars introduced in a cold and moderate area of Iran. The results showed an average polymorphism of 82.69% and 75% for anchored and non-anchored ISSR markers, respectively. This result indicates that the anchored ISSR markers have a higher rate of efficiency compared to non-anchored ISSR markers. Therefore, simultaneous grouping of cultivars with the use of anchored and non-anchored ISSR markers can differentiate the cultivars of cold and moderate regions. When anchored and non-anchored ISSR markers were simultaneously applied for cluster analysis and grouping of the individuals, the pattern of genetic diversity aligned with the pattern of geographical distribution, and the cultivars attributed to identical geographical regions were allocated in one group. Based on applied clustering with two anchored and non-anchored markers, it was concluded that Pishtaz and Mahdavi cultivars had less genetic similarity compared to other cultivars.


Blair M. W., Panaud O., and McCouch S. R. (1999). Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theoretical and Applied Genetics, 98: 780-792.

Bornet B., and Branchard M. (2001). None anchored inter simple sequence repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Molecular Biology Reporter, 19: 209-215.

Bornet B., and Branchard M. (2004). Use of ISSR fingerprints to detect microsatellites and genetic diversity in several related Brassica taxa and Arabidopsis thaliana. Hereditas, 140: 245-247.

Etminan A., Pour-Aboughadareh A., Mohammadi R., Ahmadi-Rad A., Noori A., Mahdavian Z., and Moradi Z. (2016). Applicability of start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) markers for genetic diversity analysis in durum wheat genotypes. Biotechnology & Biotechnological Equipment, 30: 1075-1081.

El-Aziz G. H. A., Ahmed S. S., El Mangoury K., and Fahmy A. H. (2016). Using different growth regulators in wheat to overcome negative effects of drought stress as one of climate change impacts and evaluation of genetic variation using ISSR. Advances in Environmental Biology, 10: 82-91.

Joshi S. P., Gupta V. S., Aggarwal R. K., Ranjekar P. K., and Brar D. S. (2000). Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theoretical and Applied Genetics, 100: 1311-1320.

Gupta M., Chyi Y-S., Romero-Severson J., and Owen J. L. (1994). Amplification of DNA markers from evolutionarily diverse genomes using single primers of si-

mple-sequence repeats. Theoretical and Applied Genetics, 89: 998–1006.

Gupta P. K., Varshney R. K., Sharma P. C., and Ramesh B. (1999). Molecular markers and their applications in wheat breeding. Plant Breeding, 118: 369-390.

Kumar S., Kumar V., Kumari P., Singh A. K., and Singh R. (2016). DNA fingerprinting and genetic diversity studies in wheat genotypes using SSR markers. Journal of Environmental Biology, 37: 319-326.

Pradeep Reddy M., Sarla N., and Siddiq E. A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9–17.

Ramshini H., Mirzazadeh T., Moghaddam M. E., and Amiri R. (2016). Comparison of old and new wheat cultivars in Iran by measuring germination related traits, osmotic tolerance and ISSR diversity. Physiology and Molecular Biology of Plants, 22: 391-398.

Saghai-Maroof M. A., Soliman K., Jorgensen R. A., and Allard R. W. (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of National Academy of Sciences, 81: 8014–8.

Sharma K., Agrawal V., Gupta S., Kumar R., and Prasad M. (2008). ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis). Plant Biotechnology Report, 2:239–243.

Sofalian O., Chaparzadeh N., Javanmardand A., and Hejazi M. S. (2008). Study the genetic diversity of wheat landraces from North west of Iran based on ISSR molecular markers. International Journal of Agriculture Biology, 10:466-468.

Tarinejad A. (2013a). Classification of closely related wheat cultivars with ISSR marker. Technical Journal of Engineering and Applied Sciences, 3: 1331-1337.

Tarinejad A. (2013b). Studies on genetic diversity between and within six wheat populations using ISSR markers. Research on Crops, 14: 37-41.

Wu K., Jones R., Dannaeberger L., and Scolnik P. A., (1994). Detection of microsatellite polymorphisms without cloning. Nucleic Acids Research, 22: 3257–3258.

Zietkiewicz, E., Rafalski A., and Labuda D. (1994). Genome fingerprinting by simple sequence repeat (SSR) – anchored polymerase chain reaction amplification. Genomics, 20: 176–183.